Is this antibody suitable for functional studies, not just binding assays?

Yes, absolutely. This human anti-MS4A1 (CD20) antibody is specifically validated for functional (FuncS) and neutralization (Neut) assays, as noted in its application list. This makes it a valuable tool for research investigating the biological function of the CD20 molecule on human cells, beyond simple detection.

What applications is TAB-772 validated for?

This antibody has a very broad validation profile. It is suitable for use in ELISA, Immunoprecipitation (IP), Flow Cytometry (FC), functional studies (FuncS), neutralization (Neut), Immunofluorescence (IF), and Immunocytochemistry (ICC). As a human IgG1 antibody, it is a versatile reagent for studying human MS4A1 (CD20).

Human Anti-MS4A1 Recombinant Antibody (TAB-772)

CAT#: TAB-772

This product is a recombinant Human antibody that recognizes MS4A1/CD20. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.

Tested Data Gene Expression

Figure 1 Anti-CD20 Antibody (TAB-772) in Flow Cytometry

Flow cytometry analysis of Daudi cells with Anti-CD20 Antibody (Cat#TAB-772, Blue line) or isotype control antibody (Yellow line). A Goat Anti-human IgG (FITC) was used as the secondary antibody.

Figure 2 Anti-CD20 Antibody (TAB-772) in WB

Western blot analysis of TAB-772 was performed with CD20 Protein, Human, Recombinant (TrxA Tag).
TAB-772 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing antigen (0.1μg)
Lane 2: Reducing antigen (0.3μg)
Lane 3: Reducing antigen (0.6μg)

SDS-PAGE

Figure 3 Human Anti-MS4A1 Recombinant Antibody (TAB-772) in SDS-PAGE

Lane 1: Non-reduced mAb
Lane 2: Reduced mAb

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Subcellular Location and Protein Expression Figure 1 IF staining of human cell line REH

Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/1778_G3_5_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/201_H5_1_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/202_H5_1_red_green.jpg

Normal Tissue Figure 4 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_3.jpg

Normal Tissue Figure 5 Bone marrow

Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_B_4_4.jpg

Normal Tissue Figure 6 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_8.jpg

RNA Expression Figure 7 RNA cell line category: Group enriched (Daudi, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156738-MS4A1

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Humanized (from mouse)

Type
IgG1

Specificity
Human MS4A1/CD20

Species Reactivity
Human

Applications
Suitable for use in ELISA, IP, FC, FuncS, Neut, IF, ICC and most other immunological methods.

Related Disease
Multiple sclerosis (MS) (relapsing remitting)

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
MS4A1;membrane-spanning 4-domains, subfamily A, member 1;CD20;B-lymphocyte antigen CD20;B1;Bp35;CD20 antigen;CD20 receptor;leukocyte surface antigen Leu-16;B-lymphocyte cell-surface antigen B

Gene ID
931

UniProt ID
P11836

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

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Breast cancer biomarkers at key points during disease progression

Excellent for Flow Cytometry

We are very pleased with this antibody (TAB-772) for detecting human MS4A1 (CD20) by flow cytometry (FC). As a human IgG1, it works well in our human cell-based systems with minimal background. The broad validation for other applications like IF and IP is a huge bonus.

Great for Functional Assays

This human anti-MS4A1 antibody is perfect for our research. We specifically needed a reagent validated for functional studies (FuncS) and neutralization (Neut), and this product lists both. Being a human IgG1, it's ideal for our in vitro functional assays on human B-cells.

Q&As

Is this antibody suitable for functional studies, not just binding assays?

A: Yes, absolutely. This human anti-MS4A1 (CD20) antibody is specifically validated for functional (FuncS) and neutralization (Neut) assays, as noted in its application list. This makes it a valuable tool for research investigating the biological function of the CD20 molecule on human cells, beyond simple detection.
What applications is TAB-772 validated for?

A: This antibody has a very broad validation profile. It is suitable for use in ELISA, Immunoprecipitation (IP), Flow Cytometry (FC), functional studies (FuncS), neutralization (Neut), Immunofluorescence (IF), and Immunocytochemistry (ICC). As a human IgG1 antibody, it is a versatile reagent for studying human MS4A1 (CD20).

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-772, RRID: AB_3112009)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-772.pdf

MSDS

MSDS - TAB-772.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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